Open Access Highly Accessed Research article

H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast

Grace S Shieh1, Chin-Hua Pan2, Jia-Hong Wu1, Yun-Ju Sun1, Chia-Chang Wang1, Wei-Chun Hsiao2, Chia-Yeh Lin2, Luh Tung3, Tien-Hsien Chang3, Alastair B Fleming5, Cory Hillyer7, Yi-Chen Lo4, Shelley L Berger6, Mary Ann Osley7* and Cheng-Fu Kao2*

Author Affiliations

1 Institute of Statistical Sciences, Academia Sinica, Academia Road, Nankang, Taipei 115, Taiwan

2 Institute of Cellular and Organismic Biology, Academia Sinica, Academia Road, Nankang, Taipei 115, Taiwan

3 Genomic Research Center, Academia Sinica, Academia Road, Nankang, Taipei 115, Taiwan

4 Institute of Food Science and Technology, National Taiwan University, Daan, Taipei 106, Taiwan

5 Microbiology, Moyne Institute of Preventive Medicine, The University of Dublin, Trinity College, College Green, Dublin2, Dublin, Ireland

6 Departments of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

7 Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA

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BMC Genomics 2011, 12:627  doi:10.1186/1471-2164-12-627

Published: 22 December 2011

Additional files

Additional file 1:

This file contains Supplementary Figure S1-S5 and Supplementary table S1-S5. The supplementary figures include: Figure S1. Validation of H2B ubiquitylation levels at various genomic regions; Figure S2. Comparison of the genomic distributions of H2BK123ub1, H3K4me3, H3K36me3, and H3K79me3; Figure S3. Comparison of the genomic distributions of H2BK123ub1 and H3K79me2/me3; Figure S4. Comparison of the regional distributions of H2BK123ub1 and H3K79me2/me3; Figure S5. H2B ubiquitylation does not interact with genes that function in U1 (MUD2), RNA export (SAC3), RNA decapping (EDC2) and RNA degradation (LSM1). The supplementary tables include: Table S1 Classification of genes by transcription rate or gene length; Table S2 Classification of highly transcribed genes by gene length; Table S3 S. cerevisiae strains; Table S4 Primers for probe based quantitative PCR; Table S5 Primers for quantitative PCR.

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