Open Access Research article

Polyphenism in social insects: insights from a transcriptome-wide analysis of gene expression in the life stages of the key pollinator, Bombus terrestris

Thomas J Colgan1*, James C Carolan12, Stephen J Bridgett3, Seirian Sumner4, Mark L Blaxter35 and Mark JF Brown16

Author Affiliations

1 Department of Zoology, School of Natural Sciences, Trinity College Dublin, Dublin 2, Ireland

2 Department of Biology, Callan Building, National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland

3 The GenePool Genomics Facility, School of Biological Sciences, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK

4 Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK

5 Institute of Evolutionary Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK

6 School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

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BMC Genomics 2011, 12:623  doi:10.1186/1471-2164-12-623

Published: 20 December 2011

Additional files

Additional file 1:

Assembly pipeline of Bombus terrestris transcriptome. This excel file contains all information regarding raw read data, primary assemblies and secondary assemblies for generation of BT_transcriptome_v2.

Format: XLS Size: 62KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Credibility testing of 454 versus Sanger data. This pdf data contains information on proportion of secondary assembly generated from 454 and/or Sanger data and in addition, credibility tests on unassembled Sanger data.

Format: PDF Size: 172KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

GoSlim analyses of BT_transcriptome_v2. This excel file contains GO terms mapped to the BT_transcriptome_v2 contig set with individual pie-charts displaying contigs annotated with molecular function (MF), biological processes (BP) and cellular component (CC) ontologies.

Format: XLS Size: 66KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Gene sets for pathways and processes across the insect groups. This excel file contains gene sets of peptide sequences generated for A. mellifera, N. vitripennis, D. melanogaster and A. pisum.

Format: XLS Size: 635KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 5:

Singletons and contigs receiving unique EST contribution from a life cycle stage. This excel file contains information on number of unique singletons (sequences with one 454 read mapped), number of unique contigs (sequences with more than one 454 read mapped), number of ESTs contributed per life cycle stage to unique contig, total number of ESTs per life cycle stage, percentage of total ESTs from each life cycle stage contributed to unique contigs and percentage of total contig set for each group of stage-restricted contigs.

Format: XLS Size: 71KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 6:

Elevated expression within the individual life cycle stages. This pdf contains information on the top ten highly expressed contigs that best distinguish a life cycle stage.

Format: PDF Size: 261KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data