Additional file 4.

Expression of miRNAs in pupal tissue. Northern blots for miRNAs in Heliconius pupal tissue collected at 24, 48 and 72 hours post-pupation (± 30 mins). H.m.m = H. melpomene melpomene, H.m.r = H. m. rosina. FW = forewing, HW = hindwing, T = thorax, U6 = U6 loading control. Gels were run for two biological replicates (A and B) for each race and stage collected.

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Surridge et al. BMC Genomics 2011 12:62   doi:10.1186/1471-2164-12-62