Open Access Research article

Characterisation and expression of microRNAs in developing wings of the neotropical butterfly Heliconius melpomene

Alison K Surridge1, Sara Lopez-Gomollon2, Simon Moxon3, Luana S Maroja14, Tina Rathjen2, Nicola J Nadeau1, Tamas Dalmay2* and Chris D Jiggins1*

Author Affiliations

1 Department of Zoology, University of Cambridge, Downing Street, Cambridge. CB2 3EJ. UK

2 School of Biological Sciences, University of East Anglia, Norwich. NR4 7TJ. UK

3 School of Computing Sciences, University of East Anglia, Norwich. NR4 7TJ. UK

4 Williams College, Department of Biology, Williamstown MA 01267. USA

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BMC Genomics 2011, 12:62  doi:10.1186/1471-2164-12-62

Published: 26 January 2011

Additional files

Additional file 1:

Heliconius miRNAs identified by deep sequencing of two colour pattern races. miRNAs identified in H. m. melpomene and H. m. rosina. Two or more miRNAs are listed when a sequence matches to two different, but related miRNAs in miRBase (the sequences and read counts of all miRNA variants detected are given in Additional files 2 and 3). Normalised abundance (number of reads per million; calculated allowing for two mismatches to mature miRNA sequences given in miRBase) is given for each race along with fold change (melpomene/rosina).

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Additional file 2:

miRNA sequences and read counts detected in H. m. melpomene. FASTA file of all identified miRNA sequence variants detected in H. m. melpomene. x refers to the number of reads obtained for each sequence.

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Open Data

Additional file 3:

miRNA sequences and read counts detected in H. m. rosina. FASTA file of all identified miRNA sequence variants detected in H. m. rosina. x refers to the number of reads obtained for each sequence.

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Open Data

Additional file 4:

Expression of miRNAs in pupal tissue. Northern blots for miRNAs in Heliconius pupal tissue collected at 24, 48 and 72 hours post-pupation (± 30 mins). H.m.m = H. melpomene melpomene, H.m.r = H. m. rosina. FW = forewing, HW = hindwing, T = thorax, U6 = U6 loading control. Gels were run for two biological replicates (A and B) for each race and stage collected.

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Additional file 5:

Identification of hme-miR-193 and hme-miR-2788 in Heliconius. Alignments of different sequences of hme-miR-193 and hme-miR-2788, their star sequences and sequence read counts to Heliconius melpomene BAC sequence.

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Additional file 6:

Probe sequences used for Northern blots. Probe sequences for ten miRNAs analysed by Northern blot.

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