Table 3

Databases used for BLAST homology searches with relevant details for each search

Reference organism

Sequence type

BLAST algorithm

Minimum e-value

Version

Source

Minimum % identity used in final EST set selection


Ae. albopictus

mRNA

BLASTN

1.00E-10

N/A

http://www.ncbi.nlm.nih.gov webcite

85

Ae. aegypti

transcripts

BLASTN

1.00E-10

AaegL1.2

http://www.vectorbase.org webcite

85

Ae. aegypti

genomic

BLASTN

1.00E-10

AaegL1

http://www.vectorbase.org webcite

85

Ae. aegypti

peptides

BLASTX

1.00E-10

AaegL1.2

http://www.vectorbase.org webcite

70

Cx. quinquefasciatus

peptides

BLASTX

1.00E-05

CpipJ1.2

http://www.vectorbase.org webcite

0

An. gambiae

peptides

BLASTX

1.00E-05

AgamP3.6

http://www.vectorbase.org webcite

0

D. melanogaster

translation

BLASTX

1.00E-05

r5.29

http://www.flybase.org webcite

0

Ca. elegans

peptides

BLASTX

1.00E-04

WS218

http://www.wormbase.org webcite

0

Swiss-Prot

proteins

BLASTX

1.00E-03

2010_6

http://www.uniprot.org webcite

0


The minimum e-value used for the initial EST set, and the minimum % identity used for the final EST set, varied based on phylogenetic distance of the organism/database and Ae. albopictus.

Poelchau et al. BMC Genomics 2011 12:619   doi:10.1186/1471-2164-12-619

Open Data