Additional file 4.

Gene accumulation curve of Ae. albopictus ESTs. The average cumulative number of recovered Ae. aegypti transcripts, plotted against the number of reads needed to obtain that number. All reads were searched against Ae. aegypti transcripts via blastn. The BLAST output order was randomized 1,000 times, and the average number of transcripts discovered with each additional read was calculated.

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Poelchau et al. BMC Genomics 2011 12:619   doi:10.1186/1471-2164-12-619