Additional file 4.

Evaluation of k-mer coverage for Velvet assembly. The frequency of k-mers (number of appearances or coverage) was determined using untrimmed Illumina reads and ESTs. The results show a large peak of k-mers with coverage of one, which mostly correspond to sequencing errors. Thus, a k-mer coverage threshold of 2 was utilized in the de novo transcriptome assembly.

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Barrero et al. BMC Genomics 2011 12:600   doi:10.1186/1471-2164-12-600