Additional file 3.

Comparison of Oases assemblies using various k-mers. A) Oases assemblies using k-mers ranging from 17 to 47 with minimum transcript size of 100 bp. B) Oases assemblies with minimum transcript size of 300 bp. C) Zoomed in view of Oases assemblies with minimum transcript size of 300 bp over k-mers 19 to 37. The maximum N50, largest number of gene clusters and largest number of transcripts can be obtained using a k-mer of 25. Thus, this k-mer and a length threshold of > = 300 bp were selected as parameters to assemble the reference E. fischeriana root transcriptome.

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Barrero et al. BMC Genomics 2011 12:600   doi:10.1186/1471-2164-12-600