Additional file 2.

Assessment of reads using FastQC after trimming. A) Quality of reads per base after adaptive window trimming using a quality average threshold of 20 and a minimum length threshold of 20, The central red line is the median value, the yellow box represents the inter-quartile range (25-75%), the upper and lower whiskers represent the 10% and 90% points and the blue line represents the mean base quality B) The mean sequence quality scores over all reads. C) The GC content distribution over all sequenced reads as compared against the theoretical GC distribution.

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Barrero et al. BMC Genomics 2011 12:600   doi:10.1186/1471-2164-12-600