CTCF binding does not change in response to estrogen or tamoxifen treatment. CTCF binding was mapped by ChIP-seq in MCF-7 cells treated with vehicle, 100 nM estrogen or 1 μM tamoxifen for 45 minutes and 3 hours. A. Heat map representing CTCF binding intensity in MCF-7 cells treated with vehicle, estrogen or tamoxifen for 45 minutes. The window represents -/+ 5 kb regions from the centre of the CTCF binding events. B. Examples of genomic loci showing CTCF binding in vehicle-, estrogen- and tamoxifen-treated MCF-7 cells. C. Global genomic distribution of CTCF binding events in MCF-7 cells.
Ross-Innes et al. BMC Genomics 2011 12:593 doi:10.1186/1471-2164-12-593