Table 3

List of mutants yielding low biomass in presence of TMP at sub-lethal concentrations in LB medium

Sensitive knock-out candidates


Gene

Gene ID

Description


deoR

b0840

Repressor for deo operon, nupG and tsx; binds deoxyribose-5-phosphate inducer

flgH

b1079

Flagellar synthesis, basal body L-ring protein

fliD

b1924

Hook-associated protein 2, axial family; flagellar regulon

fliI

b1941

Cytoplasmic membrane ATPase involved in flagellar assembly; involved in export of flagellar axial protein subunits

fliN

b1946

Flagellar switch protein

JW5799

-

mdoH

b1049

Membrane glycosyltransferase

tdk

b1238

Deoxythymidine kinase

ycbC

b0920

Function unknown

yciM

b1280

Function unknown

ydhA

b1639

Function unknown

yfgE (hda)

b2496

Required for regulatory inactivation of DnaA; multicopy suppressor of dnaN(ts)

alaS

b2697

Alanine--tRNA ligase


A total of 13 mutants, based on their relatively low yields, were identified as sensitive candidates. Genes whose functions are related to DNA maintenance and nucleotide salvage pathway are highlighted in bold. Genes are sorted by their Gene IDs.

Sangurdekar et al. BMC Genomics 2011 12:583   doi:10.1186/1471-2164-12-583

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