Open Access Research article

Human native lipoprotein-induced de novo DNA methylation is associated with repression of inflammatory genes in THP-1 macrophages

Rubén Rangel-Salazar1, Marie Wickström-Lindholm2, Carlos A Aguilar-Salinas3, Yolanda Alvarado-Caudillo1, Kristina BV Døssing5, Manel Esteller4, Emmanuel Labourier6, Gertrud Lund7, Finn C Nielsen5, Dalia Rodríguez-Ríos7, Martha O Solís-Martínez1, Katarzyna Wrobel8, Kazimierz Wrobel8 and Silvio Zaina125*

  • * Corresponding author: Silvio Zaina szaina@ugto.mx

  • † Equal contributors

Author Affiliations

1 Department of Medical Sciences, University of Guanajuato, León, Mexico

2 Experimental Cardiovascular Research Laboratory, Lund University, Malmö, Sweden

3 National Institute for Medical Sciences and Nutrition "S. Zubirán", Mexico City, Mexico

4 Cancer Epigenetics and Biology Program, IDIBELL, Barcelona, Spain

5 Center for Genomic Medicine, Rigshospitalet, Copenhagen, Denmark

6 Asuragen Inc., Austin, Texas

7 Department of Genetic Engineering, CINVESTAV, Irapuato, Mexico

8 Department of Chemistry, University of Guanajuato, Guanajuato, Mexico

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BMC Genomics 2011, 12:582  doi:10.1186/1471-2164-12-582

Published: 25 November 2011

Additional files

Additional file 1:

VLR increases intracellular lipids in THP-1 macrophages. Quantification of intracellular triglycerides and cholesterol in VLR-stimulated THP-1 macrophages. Black and grey bars, triglycerides and cholesterol, respectively. ***, P < 0.005. Data are presented as average and s.d. of triplicates of one experiment.

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Additional file 2:

Primers used in the study. List of gene-specific primers used for RT-PCR and DNA methylation analysis.

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Additional file 3:

Genes significantly regulated by VLR. Genes are ordered in descending order of expression change (VLR-stimulated vs. control cells, left column). Notice the excess of negatively regulated genes. Gene counts differ from the ones indicated in the text, as some transcripts are represented on arrays more than once.

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Additional file 4:

Clustering of genes down-regulated by VLR. Chromosomal position and cluster size for genes that are down-regulated by VLR.

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