Additional file 12.

Representative of consecutively numbered isotigs with highly similar lengths. An example of two isotigs which both have Cyclin D as their top BLAST hit (see Additional File 9), differ in length by only two nucleotides, and have highly similar sequences. Isotig07129 is 4,279 bp long; isotig07130 is 4,277 bp long. Only a portion of the sequence of each isotig is shown. Nucleotide positions differing between the two are indicated in black (likely to be SNPs), white (deletions) or grey (apparent sequence difference may be due to poor quality sequence (lower case letters) at this position).

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Zeng et al. BMC Genomics 2011 12:581   doi:10.1186/1471-2164-12-581