Diagrammatic illustration of the experimental design showing groups that were used for total RNA isolation, cDNA synthesis, array hybridization and quantitative and semi-quantitative RT-PCR validation of the array data. Numbers, 1 - 10, under each oocyte and CCs figure, represent the samples that were used for RNA isolation, cDNA synthesis and array hybridisation, as explained in materials and methods section. For all experimental groups, the samples were derived from GV stage COCs.
Regassa et al. BMC Genomics 2011 12:57 doi:10.1186/1471-2164-12-57