Open Access Research article

Transcriptome dynamics and molecular cross-talk between bovine oocyte and its companion cumulus cells

A Regassa, F Rings, M Hoelker, U Cinar, E Tholen, C Looft, K Schellander and D Tesfaye*

Author Affiliations

Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Germany

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BMC Genomics 2011, 12:57  doi:10.1186/1471-2164-12-57

Published: 24 January 2011

Additional files

Additional file 1:

The Six MIAME guidelines that were adopted to conduct the study.

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Additional file 2:

List of transcripts that are exclusively expressed in GV oocytes relative to GV CCs.

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Additional file 3:

List of transcripts that are exclusively expressed in GV CCs relative to GV oocytes.

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Additional file 4:

List of transcripts that are expressed in both GV oocytes and CCs.

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Additional file 5:

List of transcripts that are expressed only MII CCs.

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Additional file 6:

List of transcripts that are expressed in both MII oocytes and CCs.

Format: XLS Size: 366KB Download file

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Additional file 7:

List of transcripts that are differentially expressed between GV oocytes and CCs.

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Additional file 8:

List of transcripts that are expressed only in MII oocytes.

Format: XLS Size: 1.6MB Download file

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Additional file 9:

List of transcripts that are differentially expressed between MII oocytes and CCs.

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Additional file 10:

The top significantly changed GO (molecular functions) with the proportion of transcripts that are over expressed in GV oocytes and CCs (P < 0.001).

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Additional file 11:

The top significantly changed GO (molecular functions) with the proportion of transcripts that are over expressed in MII oocytes and CCs (P < 0.001).

Format: TIFF Size: 140KB Download file

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Additional file 12:

List of transcripts that are differentially expressed between oocytes cultured with or without their companion CCs.

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Additional file 13:

List of transcripts that are differentially expressed between CCs cultured with or without their oocyte.

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Additional file 14:

The seven most prominent canonical pathways involving genes that are over expressed in OO + CCs relative to OO - CCs with P-values, 0.05. The bars represent the P-value for each pathway. The orange irregular line is a graph of the ratio (genes from the data set/total number of genes involved in the pathway) for the different pathways.

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Additional file 15:

The five most prominent canonical pathways involving genes that are over expressed in CCs + OO relative to CCs - OO with P-values, 0.05. The bars represent the P-value for each pathway. The orange irregular line is a graph of the ratio (genes from the data set/total number of genes involved in the pathway) for the different pathways.

Format: TIFF Size: 62KB Download file

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Additional file 16:

List of transcripts that are exclusively expressed in GV CCs compared to MII CCs.

Format: XLS Size: 563KB Download file

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Additional file 17:

List of transcripts that are exclusively expressed in MII CCs compared with GV CCs.

Format: XLS Size: 116KB Download file

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Additional file 18:

List of transcripts that are commonly expressed between CCs at GV and MII stages.

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Additional file 19:

List of transcripts that are differentially expressed between GV and MII CCs.

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Additional file 20:

qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between GV oocytes and CCs. Transcripts marked by the minus sign indicate those over expressed in GV CCs.

Format: DOC Size: 34KB Download file

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Additional file 21:

qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between MII oocytes and CCs. Transcripts marked by the minus sign indicate those over expressed in MII CCs.

Format: DOC Size: 31KB Download file

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Additional file 22:

qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between oocytes cultured with their companion CCs relative to those cultured alone. Transcripts marked by the minus sign indicate those over expressed in oocytes cultured without their companion CCs.

Format: DOC Size: 33KB Download file

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Additional file 23:

qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between CCs cultured with their enclosed oocytes relative to those cultured alone. Transcripts marked by the minus sign indicate those over expressed in CCs cultured without their enclosed oocytes.

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Additional file 24:

qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between CCs at germinal vesicle (GV) and metaphase II (MII) stages. Transcripts marked by the minus sign indicate those over expressed in MII stage relative to GV stage.

Format: DOC Size: 31KB Download file

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