Schematic diagram of the protein binding microarray (PBM) designed to test the ability of HNF4α to bind Alu-derived DNA sequences. Top, schematic structure of a generic Alu element (~300 nt long) comprised of two related, but non identical monomers, the right and left arms (adapted from ). Box A and B are RNA Pol III internal promoters. Relative positions of the 200 Alu-derived 13-mers incorporated into PBM3 are also shown. Bottom, remaining DNA probes on PBM3 and workflow.
Bolotin et al. BMC Genomics 2011 12:560 doi:10.1186/1471-2164-12-560