Validation of CNVs using qPCR. Validation results for non-genic CNVs (panels on the left) and genic CNVs (panels on the right) are shown. Each panel is labelled with the CNV tested, and the breed assayed. The name of the overlapping gene is given in parentheses for genic CNVs. Bars represent distinct animals and are labelled with animal identifiers. The right-most bar in each panel depicts the relative copy number in a calibrator animal from the alternate breed. The calibrator is assumed to contain two copies of the DNA segment. Each bar was calculated from four technical replicates. The error bars show the minimum and maximum value encountered among the replicates.
Stothard et al. BMC Genomics 2011 12:559 doi:10.1186/1471-2164-12-559