Open Access Highly Accessed Methodology article

Functional microRNA screening using a comprehensive lentiviral human microRNA expression library

Jos B Poell1, Rick J van Haastert2, Francesco Cerisoli2, Anne S Bolijn3, Lisette M Timmer3, Begoña Diosdado-Calvo3, Gerrit A Meijer3, Andre AFL van Puijenbroek2, Eugene Berezikov1,2, Roel QJ Schaapveld2 and Edwin Cuppen1,2*

1 Hubrecht Institute, Cancer Genomics Center, and University Medical Center Utrecht. Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

2 InteRNA Technologies BV, Padualaan 8, 3585 CH Utrecht, The Netherlands

3 Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands

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BMC Genomics 2011, 12:546 doi:10.1186/1471-2164-12-546

Published: 3 November 2011

Additional files

Additional file 1:

Table S1. Contains a list of the miRNAs included in the library and their cognate genomic sequences that were cloned into the vector, and associated virus titers.

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Additional file 2:

Table S2. Contains the endogenous levels of the set of 10 test miRNAs in the five tested cell lines. Expression calculated as 2-ΔCt relative to RNU6B.

Format: XLS Size: 19KB Download file

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Additional file 3:

Figure S1. Demonstrates the lack of correlation between virus titer and toxicity.

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