Figure 2.

Melanin variants show differences in growth as well as sensitivity to fluconazole and brefeldin A. (A) Dilutions of the melanin variants were spotted on the media indicated above the panels with YNB medium as the control for growth in the presence of fluconazole (5 μg/ml) and brefeldin A (20 μg/ml). Growth on the L-DOPA plate demonstrates the difference in melanin production by the strains. The plates were incubated for 2 days at 30°C before being photographed. (B) Growth of the CBS7779 strain and three black and three white melanin variants was measured in a TECAN plate reader. The assay was repeated three times with similar results.