Table 3 |
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List of the primers used for real-time qPCR |
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|
Genes |
Accession N° |
Primers sequence (5'-3') |
|
|
|
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|
Housekeeping gene |
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|
gapdh |
Glyceraldehyde 3-phosphate dehydrogenase |
DQ403055 |
F: CAATGACCCCTTCATTGACC R: GACAAGCTTCCCGTTCTCAG |
|
Regulation of cytoskeleton |
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|
tubb2b |
β-Tubulin |
NM_023716 |
F: GCAACATGAATGACCTGGTG R: ACCAGAGACCCAGCACAAAC |
|
thy1 |
Thy1 Antigen |
NM_009382.3 |
F: AAGGCCTCTGCCTGTAGTGA R: GAAGAGGCAGGTTGCAAGAC |
|
actin |
β-Actin |
NM_007393 |
F: CACCACCACAGCCGAGAG R: CCAGGGAGGAAGAGGATGC |
|
col1a1 |
Collagen α1 |
NM_000088 |
F: GGGTCATTTCCACATGCTTT R: TCCGGGTTTCAGAGTACCAC |
|
thbs1 |
Thrombospondin 1 |
NM_011580 |
F: CCAAAGCCTGCAAGAAAGAC R: CCTGCTTGTTGCAAACTTGA |
|
plekha5 |
Plekstrin homology A5 |
NM_019012 |
F: GTGCATCTGCCTGAAGACAA R: TGGGAACCTTTAACGACTGG |
|
kif23 |
Kinesin 23 |
NM_024245.4 |
F: CCTGAGCTTTCCTGACCAAG R: AGTTCCTTCTGGGTGGTGTG |
|
has2 |
Hyaluronan synthase 2 |
NM_008216 |
F: CGGAGGACGAGTCTATGAGC R: TTTTCCGGTGTTCCAAAAAG |
|
flrt2 |
Fibronectin leucine rich 2 |
NM_201518 |
F: ACCGCACTGTGGAAGATACC R: GCAAGACAACGAGCACAAAA |
|
enah |
Enabled homolog |
NM_008680 |
F: GCCTATGCTTCAGCACTTCC R: GGGCGATTGTCTTCTGACAT |
|
dclk1 |
Doublecortin like 1 |
NM_019978 |
F: AGCCTCCACCAGCTCAGTTA R: CCATACACATCGCTCCATTG |
|
ctnnbip1 |
Catenin β interacting protein 1 |
NM_020248 |
F: TTGGCTGCAGAAAGAAACCT R: CCAGCCAATCACAACCTTTT |
|
crip1 |
Cystein rich protein |
NM_007763 |
F: AGTCCAGAGCCTGCAACCTA R: GGAGTAGCAGGGATGATTGC |
|
coro1c |
Coronin |
NM_014325 |
F: GCAGAAGAGTGGTTCGAAGG R: TGATCAGGTCGCACTTCTTG |
|
cdh3 |
Cadherin 3 |
NM_007665 |
F: CACACGACCTCATGTTCACC R: CTGTACCTCATGGCCCACTT |
|
calml3 |
Calmodulin like 3 |
NM_027416.3 |
F: ATCGACAAGGATGGAAACG R: ATCTACCTCCTCGTCGCTCA |
|
cttnbp2 |
Cortactin binding protein 2 |
NM_030249 |
F: GACAAAGAAGGCTGGACTGC R: CTCACCCACGGAAATCCTTA |
|
lrrc8a |
Leucine rich repeat 8A |
NM_177725 |
F: AGAGCCCACTTACCCCAACT R: GTGTGCAGAAGCACGAGGTA |
|
snx6 |
Sorting nexin 6 |
NM_021249 |
F: CCAAGACCTGATTTTGATGCTTC R: CATGCATCGCAACTGTCTTC |
|
nrp2 |
Neuropilin 2 |
NM_0010774 |
F: ATAAGCACTGATGTCCCACTG R: GAGTTGCTCCAATCTCCTTCA |
|
nid2 |
Nidogen 2 |
NM_008695 |
F: CTCATTCAGTTGTGCCTGC R: ATAGCTGCCTCATGACATCG |
|
Regulation of apoptosis |
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|
pi3kr1 |
phosphatidylinositol 3-kinase (p85a) |
NM_001077495 |
F: CGAGCCCGACCGGAGGTGAA R: CGCACACTGCCGTCCGAGTT |
|
bcl-2 |
B-cell lymphoma 2 |
AJ582074 |
F: CGCAGAGATGTCCAGTCAGC R: CGAACTCAAAGAAGACCACAA |
|
c-myc |
cellular myelocytomatosis oncogene |
AJ582076 |
F: GACCCTGATTCGGACCTCTT R: CGACTCCACAGCCTTCTCTC |
|
p53 |
tumor supressor p53 |
U07182 |
F: ATGACGGAAGTTGTAAGAC R: TCGGATAAGATGCTGAGG |
|
PPARs genes |
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|
ppar α |
peroxisome proliferator activated receptor alpha |
AY170844 |
F: GTTTCTTTCGGCGAACTATT R: ACACGTGAGAATCTCTGCTT |
|
ppar β/δ |
peroxisome proliferator activated receptor beta/delta |
AF486582 |
F: TGCAAGATCCAGAAGAAGAA R: GTAGATGTGCTTGGAGAAGG |
|
ppar γ |
peroxisome proliferator activated receptor gamma |
AB525757 |
F: GGACCTCTCTATGATGGATG R: GGATGCAGGTTCTACTTTGA |
|
CYP4 family genes |
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|
cyp4a17 |
Cytochrome P450 4A17 |
AJ555628 |
F: ACCAGATGCCCTACACTACC R: GTGCGTAAATGGAGAGTACA |
|
cyp4a18 |
Cytochrome P450 4A18 |
AJ555629 |
F: ACCAGATGCCCTACACTACC R: GGCCATAAATGGAGATTGCA |
|
cyp4a19 |
Cytochrome P450 4A19 |
AJ555630 |
F: ACCAGATGCCCTACACTACC R: GTGCATAAATGGAGAGTGTG |
|
|
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|
Landkocz et al. BMC Genomics 2011 12:524 doi:10.1186/1471-2164-12-524 |
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