Table 1

Chronological appearance of mutations in S. pneumoniae R6 and 1974 transformants at different levels of linezolid resistancea.

S. pneumoniae strains/transformantsb,c

LNZ MIC (μg/ml)

No. of colonies tested

spr_ rrnaDd

spr_ rrnaCd

spr_ rrnaBd

spr_ rrnaAd

spr0188e

spr0196f

spr1021g


R6

0.5

4

W

W

W

W

W

W

W

R6T1

8

4

M

W

W

M

W

W

W

R6T2

16

4

M

M

M

M

M

M

W

1974

1

4

W

W

W

W

W

W

W

1974T1

8

4

M

M

M

W

W

W

W

1974T2

16

4

M

M

M

W

W

W

W

1974T3

32

4

M

M

M

M

M

M

M


a W (wild-type version); M (1974M1 version).

b R6T1 and R6T2 are first and second level transformants, respectively, of S. pneumoniae R6 transformed with genomic DNA extracted from 1974M1.

c1974T1, 1974T2 and 1974T3 are first, second and third level transformants, respectively, of S. pneumoniae 1974 transformed with genomic DNA extracted from 1974M1.

d Mutant 1974M1 had a G2576T mutation in the four copies of spr_rrna23S.

e Mutant 1974M1 had a T409C mutation in spr0188.

f Mutant 1974M1 had a A235G mutation in spr0196.

g Mutant 1974M1 had a G-29T mutation in spr1021 (the number preceded by '-' indicates the position upstream of the ATG).

Billal et al. BMC Genomics 2011 12:512   doi:10.1186/1471-2164-12-512

Open Data