Open Access Highly Accessed Research article

Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development

Adriane Menssen15*, Thomas Häupl1, Michael Sittinger12, Bruno Delorme3, Pierre Charbord4 and Jochen Ringe12

Author Affiliations

1 Tissue Engineering Laboratory, Clinic for Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Charité Platz 1, 10117 Berlin, Germany

2 Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin Berlin, Föhrer Str. 15, 13353 Berlin, Germany

3 MacoPharma, Tourcoing, France

4 INSERM U972, University Paris 11, Le Kremlin-Bicêtre, France

5 Leibniz Institute for Molecular Pharmacology (FMP), Campus Berlin-Buch, Robert-Rössle-Str.10, 13125 Berlin, Germany

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BMC Genomics 2011, 12:461  doi:10.1186/1471-2164-12-461

Published: 24 September 2011

Additional files

Additional file 1:

Genes presenting increased expression values during adipogenesis of human MSC. Candidate genes whose mean signal expression values were increased during adipogenic development of human MSC were selected with help of SiPaGene database. The mean values of early (day 1 - day 3) or late (day 3 - day 7 - day 17 - fat) adipogenesis candidate genes are given in comparison with day 0. Affymetrix gene IDs are organized incrementally, in combination with a SD-value (SD: Standard deviation). Using SiPaGene each of the five performed MSC comparisons are working automatically with four different t-tests (see Methods). Only the strongest p-value is included for each ID and the most significant change (corresponding comparison) is highlighted in the file.

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Additional file 2:

Genes presenting decreased expression values during adipogenesis of human MSC. Candidate genes whose mean signal expression values were decreased during adipogenic development of human MSC were selected with help of SiPaGene database. The mean values of early (day 1 - day 3) or late (day 3 - day 7 - day 17 - fat) adipogenesis candidate genes are given in comparison with day 0. Affymetrix gene IDs are organized incrementally, in combination with a SD-value (SD: Standard deviation). Using SiPaGene each of the five performed MSC comparisons are working automatically with four different t-tests (see Methods). Only the strongest p-value is included for each ID and the most significant change (corresponding comparison) is highlighted in the file.

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Additional file 3:

Table S1 (increased genes) and Table S2 (decreased genes). Controlling of Affymetrix probe set annotation. Control for the correct annotation of target sequences (which is not shown) for Affymetrix probe sets shown in the Additional file 1 (increased genes) and Additional file 2 (decreased genes). Target sequences were compared to entries of the RefSeq release 46 using BLAST http://blast.ncbi.nlm.nih.gov/Blast.cgi webcite and Aligner (CodonCode Corporation). Most target sequences mapped with 100% match to the 3' end of the corresponding RefSeq entries for mRNAs.

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