Table 1

The number of mismatches to homologous synthetic miRNAs impacts assay cross-reaction tendency¤.

miRCURY

TaqMan


Number of nucleotide mismatches to synthetic miRNA*

0

1

2

3

4

0

1

2

3

4


Number of assays§

3

18

34

52

60

3

18

30

45

57

Number of false positive assays (n)#

3

17

25

38

40

2

15

22

26

33

False positive rate (false positives/number of assays) (%)

100

94

74

73

67

67

83

73

58

58


¤ A given assay can be homologous to more than one synthetic miRNA and thereby appear in more than one of the mismatch categories. This may cause the false positive rates to be over estimated.

* 0 mismatches means that only the length differs between the synthetic miRNA and the miRNA assayed.

§Number of assays with the given number of nucleotide mismatches to a miRNA in the synthetic samples.

#An assay was scored as false positive if detected at Ct < 38 (miRCURY) or Ct < 30 (TaqMan) in one or more of the synthetic samples #1 and #2.

Jensen et al. BMC Genomics 2011 12:435   doi:10.1186/1471-2164-12-435

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