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standard / ## Figure 2.
Assessment of the platforms ability to discriminate miRNAs with close sequence homology. Using the synthetic samples, with known miRNA content, it was investigated how many
of the false positives detections (miRNAs not in the sample) at a given Ct detection
threshold could be related to sequence homology to a miRNA in the sample. Plotted
are the number of false positives detected in the synthetic samples at given Ct detection
thresholds and it is indicated how many of these can be related to sequence homology.
(A, B) TaqMan analysis of synthetic samples #1 and #2. (C, D) miRCURY analysis of
synthetic samples #1 and #2. A Poisson randomness test was used to evaluate if the
fraction of homology related false positives at a given Ct detection threshold was
significantly higher than expected. The expected fraction was defined as the number
of potential false positives (the number of assays on a given platform targeting miRNAs
not present in the investigated sample) that have sequence homology to miRNAs in the
sample. As the synthetic samples #1 and #2 contain the same miRNAs (but in different
concentrations) the expected fraction is the same for the two samples. For the TaqMan
platform the expected fraction is 93 out of 565 (16%) miRNAs and for the miRCURY platform
it is 102 out of 552 (18%). **p < 0.0001, *p < 0.05.
Jensen |