The effect of PLP synthesis inhibition on cell growth and megamitochondrial formation. A. SL2 cells were seeded in a 96- well plate (2.5 × 104 cells/well) and the growth rate was examined by the MTT assay in 4-DPN-treated cells as described . Control cells were not treated with 4-DPN. Experiments were performed in triplicate, and error bars denote the standard deviation from the mean of three independent experiments. B. Three days after 4-DPN treatment, cells were stained with JC-1 and then observed under a confocal microscope as described in Methods. Control cells were grown in the absence of 4-DPN. Scale bars represent 5 μm. C. Five days after treatment of cells with dsRNAs and 4-DPN, mRNA levels of GS1 and l(2)01810 were measured by realtime RT-PCR as described in Materials and Methods. dsRNAs and 4-DPN treated are shown on the X axis. Experiments were performed in triplicate, and error bars denote the standard deviation from the mean of three independent experiments. Statistical significance was tested by one-way ANOVA followed by Tukey's multiple comparison test. *** indicates significance at p < 0.001.
Lee et al. BMC Genomics 2011 12:426 doi:10.1186/1471-2164-12-426