Table 2

Short-term vs. long-term effects of lack of PKA on repression of different genes by glucose

Strain

Growth conditions

Gene


TPS1

TPS2

HXT6/7

GRX2

PEP4


Repression factor


TPK1 TPK2 TPK3

30 min with glucose

7.7

7.1

30

9.5

15


growth in medium with glucose

1.3

1.6

68

2.9

1.8


tpk1 tpk2 tpk3

msn2 msn4

30 min with glucose

1.3

1.1

0.2

0.7

0.4


growth in medium with glucose

1.5

1.1

0.2

0.9

1


tpk1 tpk2 tpk3

yak1

30 min with glucose

1.1

1.3

1.4

1.4

1.2


growth in medium with glucose

0.3

0.4

0.3

0.5

0.6


Yeasts were grown on ethanol as described in Methods and samples were taken before the addition of glucose to the medium and 30 min after. The yeasts were also grown on a medium containing 2% ethanol and 4% glucose and collected when at least 50% of the glucose was still present in the medium. Biological duplicates were performed and mRNA levels were measured by RT-PCR as described. Expression data from biological replicates generally differed from the average by less than 25%. The repression factor is the quotient of the values measured in samples grown in the absence of glucose and in the presence of glucose.

Livas et al. BMC Genomics 2011 12:405   doi:10.1186/1471-2164-12-405

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