Table 1

Short-term vs. Long-term effects of lack of PKA on induction of different genes by glucose

Strain

Growth conditions

Gene


PDC1

PDC5

ALD5

TMT1

YMC2

ATR1


Induction factor


TPK1 TPK2 TPK3

30 min with glucose

12.8

235

5

9.7

11

13.8


growth in medium with glucose

27

1.3

3

2

4

2.3


tpk1 tpk2 tpk3

msn2 msn4

30 min with glucose

15

70

3.4

1.7

2.2

2.6


growth in medium with glucose

21

4.3

3.2

1.9

1.9

2.9


tpk1 tpk2 tpk3

yak1

30 min with glucose

1.2

1.8

0.9

1.1

0.8

1.4


growth in medium with glucose

4.4

2

0.6

0.7

0.7

0.9


For short-term experiments yeasts were grown on ethanol as described in Methods and samples were taken before the addition of glucose to the medium and 30 min after. The yeasts were also grown on a medium containing 2% ethanol and 4% glucose and collected when over 50% of the glucose was still present in the medium. Biological duplicates were performed and mRNA levels were measured by RT-PCR as described. Expression data from biological replicates generally differed from the average by less than 25%. The induction factor is the quotient of the values measured in samples grown in the presence of glucose and in the absence of glucose.

Livas et al. BMC Genomics 2011 12:405   doi:10.1186/1471-2164-12-405

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