Table 4

An overall validation of microarray data by qRT-PCR

Gene

GenBank ID

Microarray FC

qRT-PCR FC


TAP1

NM_001044581

2.46

2.83

SAA2

CB475095

8.42

2.86

C4

CF366280

2.95

3.53

CD2

NM_213776.1

2.93

6.43

IRG6

NM_213817.1

3.84

32.50

TCN1

BX675338

7.37

3.56

ISG20

NM_001005351.1

5.41

2.15

PR39

NM_214450

13.51

16.48

PMAP23

EW419136

21.7

13.07

NPG4

NM_213863

8.24

21.99

TGFB2

NM_214015.1

-2.55

-3.83

VEGFA

EU714324

-2.42

-6.20

TLR5

NM_001123202

-2.10

-5.78


Fold changes of gene expression in the porcine lung 3 days following H1N1 SwIV infection compared with the controls. The fold changes were calculated by the formula of 2-delta-delta Ct, data was means ± SD of triplicate reactions for each gene transcript. The expression level of GAPDH was assayed for normalization during quantitative PCR.

Li et al. BMC Genomics 2011 12:398   doi:10.1186/1471-2164-12-398

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