Identification of potential Caryophyllaceae-specific genes. The first step identifies sequences without known homologues in reference species; the second and the third steps select sequences that are found in at least two SiESTa libraries. Sequences that partially match repeated elements are removed. In our final step we compared the remaining sequences with the Silene EST library of Moccia et al.  to identify potential Caryophyllaceae-specific genes.
Blavet et al. BMC Genomics 2011 12:376 doi:10.1186/1471-2164-12-376