Detection of rice OsBRI1 orthologs in switchgrass. DNA from BAC clones identified by library screening with the rice BRI1 gene (OsBRI1) was used as template for PCR amplification with primers S1, S2 and S3 designed from OsBRI1 sequence (Table 2). Purified PCR products recovered from gel were sequenced to verify their identity. Example of PCR amplification for three orthologous BAC clones, 72H22 (E3), 72K21 (E4) and 92J12 (E5) from homoeologous chromosomes are shown.
Saski et al. BMC Genomics 2011 12:369 doi:10.1186/1471-2164-12-369