Genotypic variations in the expression of new miRNAs. (a) The frequency count of small RNAs for each new miRNA was used to calculate its abundance. (b) The miRNA abundances were used to calculate their relative fold change in expression between BTx623 and Rio, and between the LB/EF F2s and HB/LF F2s libraries, respectively. Positive values in the y-axis of the graph denote fold changes in miRNA expression that are higher in BTx623 relative to Rio and higher in LB/EF F2s relative to HB/LF F2s libraries, respectively; the opposite is true for negative values. The miRNA miR5383 was not included in the graph because it was not detected in the Rio library (see Additional file 9, Table S4).
Calviño et al. BMC Genomics 2011 12:356 doi:10.1186/1471-2164-12-356