Additional file 1.

Location of iTol2kan insertions (new BAC ends) in APPb:EGFP and fgf24:EGFP BACs. Newly created ends containing the iTol2kan cassette were sequenced with primers Tkan1 and Tkan2 for APPb:EGFP BAC deletions (Panel A), and with primer Seq1 for fgf24:EGFP BAC deletions (Panel B). The sequences were BLASTed to the zebrafish genome, and location of the new ends of BACs where the iTol2kan cassettes are placed indicated in panels A and B. Clone numbers are as in lanes shown by the blue arrowheads in Figure 4.

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Shakes et al. BMC Genomics 2011 12:351   doi:10.1186/1471-2164-12-351