Schematic of deletion formation in fgf24:EGFP BAC by pTnloxP-iTol2kan: Layout of the fgf24 gene functionalized with the EGFP gene is shown. Below it is a schematic of the TnloxP-iTol2kan transposon insertion into the EGFP functionalized BAC DNA to place iTol2kan at the loxP end of BAC insert. The loxP-loxP deletion mediated by Cre protein is indicated.
Shakes et al. BMC Genomics 2011 12:351 doi:10.1186/1471-2164-12-351