Open Access Highly Accessed Research article

Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and high resolution melting analysis

Yuanhong Han1, Yun Kang2, Ivone Torres-Jerez2, Foo Cheung34, Christopher D Town4, Patrick X Zhao2, Michael K Udvardi2 and Maria J Monteros1*

Author Affiliations

1 Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

2 Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

3 Center for Human Immunology, Autoimmunity and Inflammation, National Institute of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

4 The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA

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BMC Genomics 2011, 12:350  doi:10.1186/1471-2164-12-350

Published: 6 July 2011

Additional files

Additional file 1:

Characteristics of 192 SNP primer pairs developed using alfalfa 454 sequences. The file contains the primer name, SNP category, amplicon size, Tm, and primer sequences.

Format: XLSX Size: 23KB Download file

Open Data

Additional file 2:

Summary of validation results of 192 SNP in alfalfa using HRM analysis. The file contains the SNP category, number of SNP, and the SNP validation status.

Format: DOC Size: 32KB Download file

This file can be viewed with: Microsoft Word Viewer

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