Additional file 2.
Figure S2, Additional file 2. Sanger read coverage for validated SNPs. All SNPs that were found in common between r1766, r02120, and 17573 (306,782, Figure 1B) were tested for Sanger read coverage using the 17573 Sanger data by looking for the same location and allele SNP call in the Sanger output (red bars). Average genome-wide Sanger read coverage was 5.2X. We analyzed an identical number of randomly selected SNPs that were detected in only two of the three resequenced animals (blue bars) to test if there were differences in the distribution of read coverage for the two SNP data sets. The distributions are indistinguishable, however, and the proportions of tested SNPs exhibiting 11+ read coverage are not statistically different (3.8% for SNPs in all three animals, 3.6% for SNPs detected in only two animals).
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Fawcett et al. BMC Genomics 2011 12:311 doi:10.1186/1471-2164-12-311