Differential expression levels of nTARs in investigated tissues. (A) Total counts of nTARs with 3-fold or higher changes in detection levels between tissues. For nTARs joint to a known gene, expression was corrected by fold change of the related gene. While NGA show a high variability, related genes usually are also linked in expression levels of neighbouring genes (for colour code, see Figure 4A). Additionally, orientation of differentially expressed nTARs is shown, compared to the orientation of total counts (Figure 5C), sense orientated findings show reduced expression variability, especially in DGN. (B) Example for a differentially expressed nTAR. In the colon, strong expression of Coq2 (coenzyme Q2 homolog, prenyltransferase) can be observed, but only slight expression in the ELU region of the last exon. In contrast, in the small intestine we observed a lower level of transcript expression, yet the nTAR region upstream of the last exon (ELU event) is distinctly upregulated in comparison to colon samples. Investigating the orientation of involved reads (dashed box) suggests, that this ELU event is transcribed in antisense orientation to the related gene.
Klostermeier et al. BMC Genomics 2011 12:305 doi:10.1186/1471-2164-12-305