Open Access Highly Accessed Research article

De novo sequencing and characterization of floral transcriptome in two species of buckwheat (Fagopyrum)

Maria D Logacheva124*, Artem S Kasianov3, Dmitriy V Vinogradov4, Tagir H Samigullin1, Mikhail S Gelfand45, Vsevolod J Makeev367 and Aleksey A Penin248

Author Affiliations

1 Department of Evolutionary Biochemistry, A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, Russia

2 Evolutionary Genomics Laboratory, Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, Moscow, Russia

3 V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

4 A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Science, Moscow, Russia

5 Faculty of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, Moscow, Russia

6 N.I Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

7 State Scientific Institute of Genetics and Selection of Industrial Microorganisms, GosNIIgenetika, Moscow, Russia

8 Department of Genetics, Biological faculty, M.V. Lomonosov Moscow State University, Moscow, Russia

For all author emails, please log on.

BMC Genomics 2011, 12:30  doi:10.1186/1471-2164-12-30

Published: 13 January 2011

Additional files

Additional file 1:

Fagopyrum esculentum transcriptome sequence data. The sequences of F. esculentum contigs and singletons and the summary of BLAST search and GO annotation of these sequences.

Format: ZIP Size: 14.4MB Download file

Open Data

Additional file 2:

Fagopyrum tataricum transcriptome sequence data. The sequences of F. tataricum contigs and singletons and the summary of BLAST search and GO annotation of these sequences.

Format: ZIP Size: 12.4MB Download file

Open Data

Additional file 3:

Coverage of previously identified Fagopyrum esculentum genes. Accession numbers of the sequences of F. esculentum genes known from previous studies and their coverage in the 454 transcriptome assembly.

Format: DOC Size: 68KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Distribution of GO categories in PDEG. Distribution of GO categories for Biological Process (a) and Molecular Function subontologies (b) for F. esculentum (violet bars) and F. tataricum (blue bars) PDEG.

Format: JPEG Size: 2.6MB Download file

Open Data

Additional file 5:

SDM phylogenetic tree. Phylogenetic tree inferred from the SDM analysis of 13 single-copy nuclear genes in 73 seed plant taxa. Scale bar corresponds to one substitution per ten sites.

Format: JPEG Size: 1.1MB Download file

Open Data

Additional file 6:

cDNA synthesis, amplification and normalization. Detailed protocol of cDNA synthesis, amplification and normalization.

Format: DOC Size: 24KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data