Differential KIR expression between subjects with a common KIR haplotype. A) KIR genotypes obtained by pyrosequencing are shown for three rhesus macaque half-siblings. The relative expression level of each detected KIR allele is shown. Striped bars indicate alleles present on their shared KIR haplotype. The number of pyrosequencing reads is shown to the left of each graph. B) For r95061, the KIR alleles detected by cDNA cloning are shown. A total of 101 clones were examined. If indicated, the allele detected is listed. A plus sign indicates the genotyping resolution obtained by cloning is identical to the resolution obtained by pyrosequencing.
Moreland et al. BMC Genomics 2011 12:295 doi:10.1186/1471-2164-12-295