Additional file 3.

Expression of Eba-otd in ovarian follicles and early embryos. (A-C) Eba-otd in situ hybridizations of ovarian follicles (A,B) and an early embryo (C). Anterior is left, dorsal is up. (D) Increase of zygotic expression levels relative to maternal expression levels measured for Eba-otd, Eba-cad, and Eba-hb using qPCR (orange bars) and transcriptome coverage (grey bars). qPCR data are based on the average of two independent PCRs, each of which run in triplicate. Bars indicate variance between samples.

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Lemke et al. BMC Genomics 2011 12:278   doi:10.1186/1471-2164-12-278