Experimental design and effect of PCB treatment on expression of CYP1A. A. Experimental design for treatment and sampling. Killifish embryos (4 hpf) were exposed to DMSO or PCB-126 (50 nM) for 1 hr. Sampling was at 5, 10, or 15 dpf. See Methods for additional details. B. Expression of CYP1A as measured by real-time RT-PCR. Results (means ± standard deviations of 3 biological replicates) are normalized to expression of beta-actin and expressed relative to the DMSO-treated control in each group. *Statistically significant difference vs. DMSO-treated controls at the same sampling time.
Oleksiak et al. BMC Genomics 2011 12:263 doi:10.1186/1471-2164-12-263