Expression of the Hdac1 neurogenic programme is stably attenuated in Hdac1ATG1 morphant embryos. (A) 6-somite stage (12 hpf) embryos and (B) 27 hpf embryos injected with standard Control + p53 morpholinos or Hdac1ATG1 + p53 morpholinos, analysed for expression of neurod4, ascl1b, neurod, dlb, lhx9 and sox2. Expression of neurod4, ascl1b, neurod, dlb and lhx9 was reduced in Hdac1-deficient embryos. In contrast, the expression pattern of sox2, which did not exhibit a statistically significant change in transcript abundance in the microarray analysis, was unaltered in 12 hpf Hdac1ATG1 morphant embryos.
Harrison et al. BMC Genomics 2011 12:24 doi:10.1186/1471-2164-12-24