Genome-wide survey reveals dynamic widespread tissue-specific changes in DNA methylation during development
- Equal contributors
1 Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada
2 Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY, USA
3 Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY, USA
4 Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA
5 Department of Zoology, North-Eastern Hill University, Umshing Mawkynroh, Shillong, Meghalaya, India
6 Department of Bioinformatics, University of British Columbia, Vancouver, British Columbia, Canada
7 Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY, USA
8 Division of Cancer Genetics, Department of Advanced Medical Science, Nihon University School of Medicine, Tokyo, Japan
9 Division of Cancer Genetics, Chiba Cancer Center, Research Institute, Chiba, Japan
BMC Genomics 2011, 12:231 doi:10.1186/1471-2164-12-231Published: 11 May 2011
Changes in DNA methylation in the mammalian genome during development are frequent events and play major roles regulating gene expression and other developmental processes. It is necessary to identify these events so that we may understand how these changes affect normal development and how aberrant changes may impact disease.
In this study
Our data suggests the vast majority of unique sequence DNA methylation has tissue specificity, that demethylation has a prominent role in tissue differentiation, and that DNA methylation has regulatory roles in alternative promoter selection and in non-promoter regions. Overall, our studies indicate changes in DNA methylation during development are a dynamic, widespread, and tissue-specific process involving both DNA methylation and demethylation.