Research article
Detection of small RNAs in Bordetella pertussis and identification of a novel repeated genetic element
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* Corresponding author: David Hot david.hot@pasteur-lille.fr
1 Center for Infection and Immunity of Lille (CIIL), Institut Pasteur de Lille, Lille, France
2 U1019, Institut National de la Santé et de la Recherche Médicale, Lille, France
3 UMR8204, Centre National de la Recherche Scientifique, Lille, France
4 Univ Lille Nord de France, Lille, France
5 IFR142, Molecular and Cellular Medicine, Lille, France
6 Genoscreen, Campus Institut Pasteur de Lille, Lille, France
BMC Genomics 2011, 12:207 doi:10.1186/1471-2164-12-207
Published: 27 April 2011Additional files
Additional file 1:
Additional figure S1 - Additional tables S1, S2, S4 - Additional list S1. Figure S1: Preliminary Northern blot analysis. Detection of transcripts in early(E), exponential(Ex) and stationary(S) phases. Table S1: Biotin probe sequences used in northern blot analysis. Table S2: RNAz prediction details. Table S4: BRE details. List S1: Bordetelle BRE sequences and positions.
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Additional file 2:
Additional figure S2 - Additional table S3. Figure S2: Test for independent transcription between the putative sRNA positions and the upstream or downstream ORF and RT-PCR primers. Table S3: List of primers used in PCR described in figure S2.
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