Additional file 1.
Total RNA isolation and normalized cDNA library construction. Total RNA was isolated from roots (R), mature leaves (L), flowers (F), developing seeds (DS), and embryos (E) of Jatropha curcas (Figure A). Normalized cDNA library was constructed from pooled total RNA and the cDNA inserts were PCR amplified from 10 randomly selected clones and resolved in 1.0% agarose gel electrophoresis with 1.0 kb DNA size markers (Figure B).
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Natarajan and Parani BMC Genomics 2011 12:191 doi:10.1186/1471-2164-12-191