Open Access Highly Accessed Research article

Genome-wide identification and expression profiling of auxin response factor (ARF) gene family in maize

Hongyan Xing12, Ramesh N Pudake12, Ganggang Guo12, Guofang Xing12, Zhaorong Hu12, Yirong Zhang13, Qixin Sun12* and Zhongfu Ni12*

Author Affiliations

1 State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China

2 National Plant Gene Research Centre (Beijing), Beijing, 100193, China

3 National Maize Improvement Center, Beijing, 100193, China

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BMC Genomics 2011, 12:178  doi:10.1186/1471-2164-12-178

Published: 7 April 2011

Additional files

Additional file 1:

Primer sequences for full-length cDNA cloning of 13 ZmARFs.

Format: DOC Size: 38KB Download file

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Additional file 2:

Sequence identity matrix of maize ARF proteins. BioEdit program were employed to examine sequence identity of 31 maize ARF proteins.a

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Additional file 3:

Sequence alignment maize ARF proteins. Clustal_X program were employed to examine sequence features of 31 maize ARF domains.

Format: RTF Size: 2.7MB Download file

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Additional file 4:

miR160, 167 and TAS3 target site prediction for ZmARF genes.

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Additional file 5:

Promoter analysis of maize ARFs. Auxin response elements are shown in the list.

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Additional file 6:

The relative expression levels of 31 ZmARFs in three tissues of maize inbred line B73. Leaves (8-day-old seedling), roots (8-day-old seedling) and embryos (15d after pollination) of maize inbred line B73 were used for real-time RT-PCR analysis.

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Additional file 7:

Primers used for stability evaluation of housekeeping gene expression.

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Additional file 8:

Stability evaluation of housekeeping gene expression. Auxin treated 8-day-old primary roots (A) and embryos during seed development and germination (B) were used for evaluating the stability of five housekeeping genes.

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Additional file 9:

Primers used for expression study of ZmARF genes.

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