Annotation of S-LAPs on 2D gels of purified sperm. Gels were run using duplicate samples of solubilized sperm protein (100 ug/gel). Proteins were first separated on isoelectric focusing gels with pH ranges of (A) 4-7, (B) 6-11 and (C) 3-10 followed by second dimension SDS-PAGE using 12% Bis-Tris reducing gels. Spots were visualized using Coomassie Blue R250 and spots excised and submitted for MALDI-TOF and tandem MS/MS analyses. The α- and β-tubulins are indicated in (A) at the expected pI and molecular weight positions. Regions of the gel identified as S-LAPs are indicated by brackets with individual spots circled.
Dorus et al. BMC Genomics 2011 12:177 doi:10.1186/1471-2164-12-177