Standard deviation of commonly used and novel reference genes. Standard deviation (SD) of gene expression of commonly used reference genes (A) across all samples (a) or across subsets of tissue-specific samples (b) from the AffymetrixMouse430 2.0 array dataset. In (B), for each subset of tissue-specific samples, the most stable genes were identified using Genevestigator RefGenes. Their respective expression SD across all arrays (c) or across subsets of tissue-specific arrays (d) is shown. The control reference genes used in this study and shown in plot A were: HSP90AB1, TFRC, B2 M, NONO, GUSB, UBC, ACTB, H2AFZ, POLR2A, TUBB4, HIST2H2AA1, RPL22, GAPDH, YWHAZ, CANX, CYC1, SDHA, EIF4A2, ATP5B, and EEF1E1.
Hruz et al. BMC Genomics 2011 12:156 doi:10.1186/1471-2164-12-156