PiggyBac insertions in cloned parasites with transient or stable expression of transposase. A. PiggyBac insertions as shown by FIGE analysis of separated chromosomes hybridized with the pbdhfr/ts or hdhfr probe. The pbdhfr/ts probe recognizes the inserted constructs, the endogenous dhfr/ts gene on chromosome 7 and the transposase construct pL1307 integrated into chromosome 5 (arrows). Without stable expression of transposase a single insert is detected in parasite clones both before and after prolonged periods of multiplication (i.e. after mechanical (am) passage; see B. In parasite clones that stably express transposase multiple inserts are detected in different chromosomes, both before and after mechanical passage (see B). B. Number of piggyBac inserts as determined by TAIL PCR in cloned parasite lines before and after prolonged periods of multiplication (i.e. after mechanical passage). ND: No data.
Fonager et al. BMC Genomics 2011 12:155 doi:10.1186/1471-2164-12-155