Table 5

Enzymes involved in TAG biosynthesis and catabolism identified by annotation of the D. tertiolecta transcriptome

Enzyme

Symbol

EC Number

Number of transcripts

1%Sequence alignment with corresponding enzymes in model organisms (Accession #)


C. reinhardtii

V. carteri


TAG biosynthesis


Glycerol kinase

GK

2.7.1.30

16

42 (A8IYH1)

NM

Glycerol-3-phosphate O-acyltransferase

GPAT

2.3.1.15

0

2NM

NM

1-Acyl-sn-glycerol-3-phosphate O-acyltransferase

AGPAT

2.3.1.51

2

NM

NM

Phosphatidate phosphatase

PP

3.1.3.4

1

NM

NM

Diacylglycerol O-acyltransferase

DGAT

2.3.1.20

2

NM

NM

Phospholipid:diacyglycerol acyltransferase

PDAT

2.3.1.158

1

NM

NM


TAG catabolism

NM

NM


Triacylglycerol lipase

TAGL

3.1.1.3

2

NM

NM


1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.

2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.

Rismani-Yazdi et al. BMC Genomics 2011 12:148   doi:10.1186/1471-2164-12-148

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