Table 4

Enzymes involved in fatty acid biosynthesis and metabolism identified by annotation of the D. tertiolecta transcriptome

Enzyme

Symbol

EC Number

Number of transcripts

1%Sequence alignment with corresponding enzymes in model organisms (Accession #)


C. reinhardtii

V. carteri


Fatty acid biosynthesis


Biotin carboxylase

BC

6.3.4.14

3

2NM

46 (EFJ41621.1)

Acetyl-CoA carboxylase

ACC

6.4.1.2

7

28 (XP_001700442.1)

28 (EFJ46223.1)

malonyl-CoA-ACP transacylase

MAT

2.3.1.39

4

NM

62 (EFJ49352.1)

Beta-ketoacyl-ACP synthase I

KAS I

2.3.1.41

7

NM

NM

Beta-ketoacyl-ACP synthase II

KAS II

2.3.1.179

12

NM

NM

Beta-ketoacyl-ACP synthase III

KAS III

2.3.1.180

1

NM

NM

Beta-ketoacyl-ACP reductase

KAR

1.1.1.100

7

63 (XP_001703473.1)

61 (EFJ52014.1)

3R-hydroxyacyl-ACP dehydrase

HAD

4.2.1.-

2

NM

NM

Enoyl-ACP reductase (NADH)

EAR

1.3.1.9

5

NM

NM

Oleoyl-ACP thioesterase

OAT

3.1.2.14

1

NM

NM

Acyl-ACP thioesterase A

FatA

3.1.2.14, 3.1.2.-

1

NM

NM

Acyl-ACP thioesterase B

FatB

3.1.2.14, 3.1.2.-

0

NM

NM


Fatty acid desaturation


Δ9 Acyl-ACP desaturase

AAD

1.14.19.2

5

76 (XP_001691597.1)

76 (EFJ49192.1)

Δ126)-Desaturase

Δ12D

1.14.19.6

3

54 (XP_001691669.1), 47 XP_001693068.1)

54 (XP_002955859.1), 47 (XP_002949932.1)

Δ153)-Desaturase

Δ15-D

1.14.19.-

2

NM

NM


Fatty acid elongation


3-Hydroxyacyl-CoA dehydrogenase

CHAD

1.1.1.35

9

NM

NM

Long-chain-3-hydroxyacyl-CoA dehydrogenase

LCHAD

1.1.1.211

2

NM

NM

Enoyl-CoA hydratase

ECH

4.2.1.17

16

NM

70 (EFJ49010.1)

Trans-2-enoyl-CoA reductase (NADPH)

TER

1.3.1.38

3

90 (XP_001690095.1)

NM

Palmitoyl-CoA hydrolase

PCH

3.1.2.22

1

19 (XP_001698944.1)

19 (EFJ40725.1)


Fatty acid catabolism


Long-chain acyl-CoA synthetase

ACSL

6.2.1.3

3

77 (XP_001693692.1)

77 (EFJ51208.1)

Acyl-CoA oxidase

AOx

1.3.3.6

2

39 (XP_001699193.1)

NM

Acyl-CoA dehydrogenase

ACADM

1.3.99.3

16

45 (XP_001699193.1), 84 (XP_001693484.1), 73 (XP_001695945.1)

NM

Enoyl-CoA hydratase

ECH

4.2.1.17

16

NM

70 (EFJ49010.1)

Long-chain 3-hydroxyacyl-CoA dehydrogenase

LCHAD

1.1.1.211

2

NM

NM

Acetyl-CoA acyltransferase

ACAT

2.3.1.16

1

NM

NM

3-hydroxyacyl-CoA dehydrogenase

CHAD

1.1.1.35

9

NM

NM

Acetyl-CoA C-acetyltransferase

thiL

2.3.1.9

6

39 (XP_001694888.1)

34 (EFJ47048.1), 34 (EFJ39622.1), 34 (EFJ46961.1)

3-hydroxybutyryl-CoA epimerase

5.1.2.3

8

NM

NM

Enoyl-CoA isomerase

ECI

5.3.3.8

8

NM

NM

Alcohol dehydrogenase

ADH

1.1.1.1

3

80 (XP_001693934.1)

53 (EFJ45245.1)

Aldehyde dehydrogenase (NAD+)

ALDH

1.2.1.3

3

24 (XP_001696928.1)

21 (EFJ40853.1)

Ferredoxin-NAD+ reductase

FNR

1.18.1.3

1

NM

NM


1In cases where multiple transcripts have been aligned with the associated enzymes in the model organisms, average similarity is reported.

2NM denotes that the annotated transcripts did not match the sequence of corresponding enzyme in model organisms.

Rismani-Yazdi et al. BMC Genomics 2011 12:148   doi:10.1186/1471-2164-12-148

Open Data